ATP requirements and small interfering RNA structure in the RNA interference pathway. Describes the crystal structure of a PIWI domain in complex with RNA. Structural insights into mRNA recognition from a PIWI domain-siRNA guide complex. The RNA-induced silencing complex is a Mg 2+-dependent endonuclease. RISC is a 5′ phosphomonoester-producing RNA endonuclease. The first crystal structure of a complete Argonaute protein, revealing its function as the catalytic component of RISC. Crystal structure of Argonaute and its implications for RISC slicer activity. Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity. References 18 and 19 report the crystal structure of the PAZ domain in complex with nucleic acids (reference 19) and mini-siRNA (reference 18). Nucleic acid 3′-end recognition by the Argonaute2 PAZ domain. Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain. Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain. Structure and conserved RNA binding of the PAZ domain. The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Structural basis for 5′-end-specific recognition of guide RNA by the A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage. A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe. Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi. Establishment and maintenance of a heterochromatin domain. On the origin and functions of RNA-mediated silencing: from protists to man. Describes a class of Argonaute proteins that do not contain the important amino acids for endonuclease activity but are still important for the RNAi pathway.Ĭerutti, H. The first exhaustive study of Argonaute genes in C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Intra- and intercellular RNA interference in Arabidopsis thaliana requires components of the microRNA and heterochromatic silencing pathways. References 1–3 are seminal works that originally described the Argonaute gene family.ĭunoyer, P., Himber, C., Ruiz-Ferrer, V., Alioua, A. A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary. AGO1 defines a novel locus of Arabidopsis controlling leaf development. Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis. Moussian, B., Schoof, H., Haecker, A., Jurgens, G.
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